Description
10-180 kDa Prestained Protein Ladder
Cat. No. PL0500
Size: 500 μll
• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15 well or 10 well mini-gel respectively.
• 1.5~2.5 μl per well for general Western transferring.
• Apply more for thicker (> 1.5 mm) or larger gel.
Introduction
The 10-180kDa Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The 10-180kDa Prestained Protein Ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes(PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.
Contents
Approximately 0.2~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25°C), 2% SDS, 1 mM 2-Mercaptoethanol, 3.6 M Urea, and 15% (v/v) glycerol).
Quality Control
5 μl of 10-180kDa Prestained Protein Ladder resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.
Note
The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each
electrophoresis condition.
For research use only.
