Description
FB No EtBr DNA 6X Loading Dye
Cat. No. DD0001
Package: 1 ml / vial
Introduction
The FB No EtBr DNA 6X Loading Dye is a non-mutagenic fluorescent reagent that enables instant visualization of agarose or acrylamide-bound DNA bands upon exposure of Blue Light or UV. The dye has an extraordinary sensitivity for DNA at <1 ng, which makes it the most sensitive stain available; its ability to work with blue light also significantly limits UV-caused damages to gel-extracted DNA. Three tracking dyes are contained (Bromophenol Blue, Xylene Cyanol FF, and Orange G) for easy visual tracking of the DNA migration progress during electrophoresis. All in all, the FB No EtBr DNA 6X Loading Dye is an ideal replacement for EtBr in terms of cost effectiveness, safety, environmental friendliness, sensitivity and convenience. The FB No EtBr DNA 6X Loading Dye is a non-mutagenic fluorescent reagent that enables instant visualization of agarose or acrylamide-bound DNA bands upon exposure of Blue Light or UV.
The dye has an extraordinary sensitivity for DNA at <1 ng, which makes it the most sensitive stain available; its ability to work with blue light also significantly limits UV-caused damages to gel-extracted DNA. Three tracking dyes are contained (Bromophenol Blue, Xylene Cyanol FF, and Orange G) for easy visual tracking of the DNA migration progress during electrophoresis. All in all, the FB No EtBr DNA 6X Loading Dye is an ideal replacement for EtBr in terms of cost effectiveness, safety, environmental friendliness, sensitivity and convenience.
Applications
1. Vortex No EtBr DNA 6X Loading Dye for 10 seconds prior to use.
2. Dilute 1 part No EtBr DNA 6× Loading Dye with 5 parts DNA sample and mix. Note: No EtBr DNA 6× Loading Dye must be added
to DNA markers in order to visualize the ladder bands simultaneously with the sample after electrophoresis.
3. Load sample and run according to standard procedures.
4. After the electrophoresis, remove gel and place on UV or a visible light transilluminator to immediately visualize bands.
5. Gels can be post-stained with Ethidium Bromide if desired.
- Store at 4°C up to 12 months. For longer periods, store at -20°C.
- No EtBr DNA 6X Loading Dye is light sensitive and should be stored protected from light.
- For Research Use Only.
