Description
FB One-Step RT-PCR
Cat. No. OS0050
Size: 50 reactions
Description
The One-Step RT-PCR system is designed for carrying out convenient, sensitive, and reproducible detection and analysis of RNA molecules by RT-PCR. Components for both cDNA synthesis and PCR are combined in a single tube, using gene-specific primers and target RNAs from either total RNA or mRNA. Reverse transcription automatically follows PCR cycling without additional steps. The system consists of two major components: RT/ HotStar Taq Mix and 2X Reaction Mix. The RT/ HotStar Taq Mix contains a mixture of Reverse Transcriptase and HotStar Taq DNA Polymerase for optimal cDNA synthesis and PCR amplification. The 2X Reaction Mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg2+ optimized for universal use,deoxyribonucleotide triphosphates, and stabilizers.
Components (50-rxn kit)
- RT/ HotStar Taq Mix–50 μl
- 50 mM Magnesium Sulfate–200 μl
- 2X Reaction Mix (contain 0.4 mM of each dNTP, 6 mM MgSO4)–1.25 ml
Store at -20°C
For research use only
- Convenient and Sensitive Detection/Analysis of RNA Molecules by RT-PCR
- Combines Reverse Transcriptase and HotStar Taq
- Optimal cDNA Synthesis and PCR Amplification in one Single Reaction
