T4 DNA Ligase
Cat. No. TL0100 Size: 100 units
Conc. 1 U/ml
Description
T4 DNA Ligase can be used to join DNA fragments with staggered or blunt ends and to repair nicks in double- stranded DNA having 3’-hydroxyl and 5’-phosphate ends.
Components
T4 DNA Ligase and 5X DNA Ligase Reaction Buffer
Unit Definition
One (Weiss) unit catalyzes the exchange of 1 nmol 32PPi into [γ/β-32P]ATP in 20 minutes at 37°C.
Note: T4 DNA Ligase is unstable on ice for long periods. Therefore, FairBiotech recommends the enzyme be kept at -20°C until within 5-10 minutes of use and returned IMMEDIATELY to -20°C after use.
Store at -20°C
For research use only
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